E Reverse transcription, cDNA amplification, and NGS library planning were executed as while in the common Fall-seq strategy. f Sequencing was carried out employing an Illumina 75 bp NGS system. “Study one�?is made up of details about the cell barcode and UMI; “Read two�?includes information about the transcript. g Facts https://www.quora.com/What-reagent-kits-are-available-for-nucleic-acid-extractions-and-how-do-they-work/answer/Even-Sa?prompt_topic_bio=1